$npx -y skills add K-Dense-AI/scientific-agent-skills --skill ginkgo-cloud-labSubmit and manage protocols on Ginkgo Bioworks Cloud Lab (cloud.ginkgo.bio), a web-based interface for autonomous lab execution on Reconfigurable Automation Carts (RACs). Use when the user wants to run protein expression and purification (cell-free, E. coli, or Pichia), HiBiT or
| 1 | # Ginkgo Cloud Lab |
| 2 | |
| 3 | ## Overview |
| 4 | |
| 5 | Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments. |
| 6 | |
| 7 | The platform also includes **EstiMate**, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols. |
| 8 | |
| 9 | The catalog is organized into **Expression & Purification** (in vitro / cell-free / E. coli / Pichia), **Characterization & Assay**, **Method & Target Onboarding**, and **Specialty**. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details. |
| 10 | |
| 11 | ## Available Protocols |
| 12 | |
| 13 | ### Expression & Purification - In vitro |
| 14 | |
| 15 | | Protocol | Readout | Price | Turnaround | Status | |
| 16 | |---|---|---|---|---| |
| 17 | | [IVT mRNA/circRNA Synthesis](references/ivt-rna-synthesis-qpcr.md) | qPCR (mRNA or circRNA, 384-well) | $99/sample | up to 12 business days | Certified | |
| 18 | |
| 19 | ### Expression & Purification - Cell-free (E. coli CFPS) |
| 20 | |
| 21 | | Protocol | Readout | Price | Turnaround | Status | |
| 22 | |---|---|---|---|---| |
| 23 | | [Validate sequence expression](references/cell-free-protein-expression-validation.md) | Go/no-go titer + purity (up to 1800 bp) | $39/sample | up to 10 days | Certified | |
| 24 | | [Optimize expression conditions](references/cell-free-protein-expression-optimization.md) | DoE across 24 conditions | $199/sample | up to 11 days | Certified | |
| 25 | | [Express + quantify (HiBiT)](references/cell-free-protein-expression-hibit.md) | Luminescence, no purification | $39/sample | up to 11 days | Certified | |
| 26 | | [Express + purify (A280)](references/cfps-strep-tag-purification-a280.md) | Strep-tag, A280 yield | $149/sample | up to 11 days | Certified | |
| 27 | | [Express + purify minibinder](references/minibinder-strep-tag-a280.md) | Strep-tag, A280, LabChip | $149/sample | up to 11 days | Certified | |
| 28 | | [Express + purify (A280 + LabChip)](references/cfps-expression-purification-quantification.md) | Strep-tag, A280 + purity/size | $159/sample | up to 12 days | Certified | |
| 29 | |
| 30 | ### Expression & Purification - E. coli |
| 31 | |
| 32 | | Protocol | Readout | Price | Turnaround | Status | |
| 33 | |---|---|---|---|---| |
| 34 | | [Express + quantify (HiBiT)](references/ecoli-protein-expression-hibit.md) | Luminescence (up to 384 constructs) | $79/sample | up to 3 weeks | Certified | |
| 35 | | [Express + purify (A280)](references/ecoli-protein-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified | |
| 36 | | [Express + purify minibinder](references/ecoli-minibinder-expression-histag-a280.md) | His-tag, A280 yield | $199/sample | up to 3 weeks | Certified | |
| 37 | | [Express + purify (A280 + LabChip)](references/ecoli-expression-purification-quantification.md) | His-tag, A280 + purity/size | $209/sample | up to 3 weeks | Certified | |
| 38 | |
| 39 | ### Expression & Purification - Pichia |
| 40 | |
| 41 | | Protocol | Readout | Price | Turnaround | Status | |
| 42 | |---|---|---|---|---| |
| 43 | | [Express + quantify (LabChip)](references/pichia-protein-expression-labchip.md) | Secreted protein, size/purity (up to 96) | $89/sample | up to 4 weeks | Certified (New) | |
| 44 | |
| 45 | ### Characterization & Assay |
| 46 | |
| 47 | | Protocol | Readout | Price | Turnaround | Status | |
| 48 | |---|---|---|---|---| |
| 49 | | [Express + thermal shift](references/cfps-strep-purification-thermal-shift.md) | SYPRO Orange Tm (Tonset, TM1-3) | $159/sample | up to 12 days | Certified | |
| 50 | | [Detect enzymatic products (Echo-MS)](references/echo-ms-cfps-detection.md) | Substrate/product by Echo-MS | $44/sample | up to 13 days | Beta | |
| 51 | |
| 52 | ### Method & Target Onboarding |
| 53 | |
| 54 | | Protocol | Readout | Price | Turnaround | Status | |
| 55 | |---|---|---|---|---| |
| 56 | | [Onboard Echo-MS method](references/echo-ms-method-onboarding.md) | Calibration curve, LOD/LOQ | $799/molecule | up to 3 weeks | Certified | |
| 57 | | [Onboard SPR target](references/spr-target-onboarding.md) | Validated SPR capture method | $1,399/target | up to 4 weeks | Beta | |
| 58 | |
| 59 | ### Specialty |
| 60 | |
| 61 | | Protocol | Readout | Price | Turnaround | Status | |
| 62 | |---|---|---|---|---| |
| 63 | | [Generate fluorescent pixel art](references/fluorescent-pixel-art-generation.md) | UV photo, 7-color E. coli palette | $25/plate | up to 7 days | |