primer-wetlab-qc

Name: primer-wetlab-qc
Author: KevinSpringer1

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How to install primer-wetlab-qc?

kevinspringer1/conserved-primer-design/primer-wetlab-qc

$npx -y skills add KevinSpringer1/conserved-primer-design --skill primer-wetlab-qc

Installs into the current project.

Summary

Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.

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SKILL.md

1	# Primer Wet-Lab QC
2
3	Use this skill after `primer_site` artifacts or external candidate generation. The bundled helper is dependency-free and conservative; final release should also run Primer3/ntthal or an equivalent thermodynamic tool when installed.
4
5	```bash
6	python agentskill/scripts/primer_panel_qc.py qc-primers \
7	--primers primer_pairs.csv \
8	--output primer_qc.json \
9	--csv-output primer_qc.csv \
10	--min-primer-len 18 \
11	--max-primer-len 30 \
12	--min-tm 50 \
13	--max-tm 68 \
14	--max-degeneracy 16
15	```
16
17	Review all pairs with:
18
19	- `degeneracy_too_high`;
20	- `three_prime_*_complement_high`;
21	- `pair_complement_run_high`;
22	- `amplicon_length_out_of_range`;
23	- large Tm differences between forward and reverse primers.
24
25	Treat `primer_qc.json` as a design gate before specificity screening and panel export.

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