kevinspringer1/conserved-primer-design
11 skills
$npx skills add kevinspringer1/conserved-primer-designSkillInstalls
advanced-phylogenyUse when an agent needs a stricter or publication-grade phylogeny workflow for PhyloGuide, including tree/MSA validation, FastTree/IQ-TREE planning, and external-tree checks.agentskillUse when an agent needs to call the VirusPrimerPro Agent API instead of the GUI, or prepare viral genome inputs for it: request/use API keys, download viral genomes from NCBI, install/run MAFFT, checkliterature-taxonomy-curationUse when an agent must define viral genotype, subtype, clade, lineage, target/background groups, or PhyloGuide groups from cited literature before primer or tNGS panel design.panel-interaction-balancingUse when an agent must split tNGS or multiplex primer pairs into pools while minimizing primer-primer interaction risk and reporting pool overflows.primer-wetlab-qcUse when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.reference-dataset-curationUse when an agent must select, filter, deduplicate, and document NCBI/GenBank/reference viral genomes before alignment, primer design, PhyloGuide, or tNGS panel design.run-orchestrationUse when an agent must batch-submit, poll, summarize, retry, or compare long-running VirusPrimerPro Agent API jobs without holding a long HTTP connection open.specificity-screeningUse when an agent must screen primer candidates or tNGS panel primers for host, near-neighbor, background, or co-infection off-target binding before reporting or ordering primers.tngs-panel-designUse when an agent must turn primer pair candidates into a tiled or multiplex tNGS amplicon panel with target coverage, overlap, selected pairs, and uncovered gap reporting.validation-reportUse when an agent must combine taxonomy, accession manifest, specificity, primer QC, and panel reports into a reproducible design release report.wetlab-exportUse when an agent must convert selected and pooled primer pairs into order sheets, pooling plans, and 96-well plate maps for wet-lab handoff.